Immunoglobulin M regulates airway hyperresponsiveness independent of T helper 2 allergic inflammation.

This is RNA sequencing data from mouse lungs of WT (Balb/C) and IgM KO (also on Balb/C background) mice that were sensitised with HDM 1ug and challenged with HDM 3ug. After mRNA isolation and conversion to cDNA and PCR amplification to give a final library, DNA nanoballs (DNBs) were generated with the ssDNA circle by rolling circle replication (RCR) to enlarge the fluorescent signals at the sequencing process. The DNBs were loaded into the patterned nanoarrays and pair-end reads of 100 bp were read through on the DNBseq platform for the following data analysis study. For this step, the DNBseq platform combines the DNA nanoball-based nanoarrays and stepwise sequencing using Combinational Probe-Anchor Synthesis Sequencing Method. The ribosomal RNA (rRNA) was first removed using SortMeRNA. We then did the Fastq file quality control using Fastqc and multiqc software to assess the quality of the raw reads followed by adapter trimming using Trim galore. The reads were then aligned to the mouse reference genome (mm10_UCSC_20180903) using STAR aligner. The map read counts was then extracted using featurecounts.