Single cell gene expression profile of hPSC-derived nephric duct spheroids (day 7) and collecting duct organoiods (day 18)

We performed directed differentiation of human pluripotent stem cells (hPSCs) into ureteric bud (UB) organoids and performed single cell transcriptomics to analyze different stages of development. At day 7, the spheroids exhibited a nephric duct (ND) signature, and we identified there is also a population of stromal progenitor cells within the cluster. At day 18, the organoids comprise more differentiated collecting duct (CD) cell types including principal cells. Overall design: During directed differentiation, structures at different timepoints were collected and dissociated into single cells for library preparation using the BD Rhapsody platform. Numerous spheroids/organoids were pooled for for analysis at each developmental stage.