Efficiency of the mini-F vector transposition in <i>E. coli</i> and the vector self-excision upon virus reconstitution.

aColony-PCR screening for determination of the mini-F excision from the US unique AvrII site. bConfirmation of the mini-F transposition into the given target site by RFLP and PCR or sequencing analysis. cOnly the repair of the unique AvrII site was investigated via sequencing. dAverage number of the last virus passage in which the mini-F sequences were detected by PCR after virus reconstitution. Numbers of the performed independent transfection experiments are given in brackets. eDetection of mini-F until passage 7 and beyond. fConfirmation of the mini-F excision in derived virus progenies by PCR or sequencing.