Additional file 3: of Guide RNAs with embedded barcodes boost CRISPR-pooled screens

Table S4. Results of the human genome-scale sgRNAiBAR library screening at different MOI for the cytotoxicity of TcdB using conventional MAGeCK analysis. Table S5. Results of the human genome-scale sgRNAiBAR library screening for the cytotoxicity of 6-TG using MAGeCKiBAR analysis. Table S6. Results of the human genome-scale sgRNAiBAR library screening for the cytotoxicity of 6-TG using conventional MAGeCK analysis. Table S7. Top 20 gene list of two biological replicates using MAGeCKiBAR and MAGeCK analysis. Table S8. sgRNA design for the functional validation of candidate genes from 6-TG screening and sgRNA design for the test of iBAR effects on activity. Table S9. Primers used for PCR amplification of the genomic DNAs and for library construction. Table S10. Comparison of the numbers of cells required for CRISPR library construction for TcdB screenings at different MOIs. (XLSX 12014 kb)