The AAV2.7m8 capsid packages a higher degree of heterogeneous vector genomes than the AAV2

In this report, we found that AAV2.7m8 packages vector genomes with a higher degree of heterogeneity than AAV2. The AAV samples were purified vector genomic DNAs and were processed for single molecule, real-time (SMRT) sequencing.Libraries were pooled and purified using 1.8x AMPure beads. Sequencing was performed on a Sequel IIe instrument. Subreads from each library underwent preprocessing steps prior to analysis. Briefly, subreads were first processed through recall adapters 9.0.0 with the following parameters: --minSnr = 2.0 and --disableAdapterCorrection. This step recovered long palindromic sequences that were artificially cleaved from subreads generated by SMRT Link (v12). Subsequent subreads were then processed using the circular consensus sequencing (CCS) tool in SMRT Link version 12.0.0 with the following parameters: --minSnr=3.75, --min=passes=2.0, and --by strand.