circRNA sequencing facilitates quantitative analysis of CUS mice hippocampus and Control mice hippocampus

The goal of this study is to seek the potential circular RNAs (circRNAs), which express differently between control group and chronic unpredictable stress (CUS) group mouse hippocampus, involved in major dipresssion disorder (MDD) by high-throughput sequencing. QPCR was preformed to identify the circRNAs trend. CircRNAs are a class of RNAs in the eukaryotic transcriptome. The function and explicit downstream mechanisms of circRNA regulated in maior depression disorser remain unclear. To further investigate the potential role of circRNAs in MDD, we performed human and mouse homology screening on circRNA sequences using the Basic Local Alignment Search Tool on the NCBI website. CircSTAG1, a homologous human and mouse circRNA, was found downregulated in circRNA high-throughput sequencing. Our study represents the first detailed analysis of circRNA expressing differently in CUS mouse hippocampus by high-throughput sequencing. CUS induced circular RNA expression in mice hippocampus was measured, and their expression in healthy controls were set as comparison. Three independent samples from CUS group and three independent samples from control group were employed in high-throughput sequencing analysis.