Mechanistic study of Wee1 kinase inhibition with AZD1775 exposes drug targetable vulnerabilities in acute B-lymphoblastic leukemia [scRNA-seq]

This study characterized the effect of WEE1 kinase inhibition using AZD1775 treatment on single-cell gene expression profile using the 10x Genomics protocol in the acute lymphoblastic leukemia cell lines RS4;11 (KMT2A-rearranged subtype) and Nalm6 (other subtype). scRNA-seq samples were generated from 24 h after AZD1775 using the 10X Genomics Chromium platform and protocols. DMSO treated cells were used as controls. Parallel cell cultures from RS4;11 cells were processed for scATAC-seq.