Statistical analysis of effects of glatiramer acetate (GA) on thrombin-induced CD62P upregulation and platelet aggregate formation for human and mouse platelets¹.

1Human or mouse platelets were stimulated with thrombin in the presence of GA vs. Control (PBS) as described in Methods. 2Optimal concentration of GA is 100 µg/ml for human platelets. 3Optimal concentration of GA is 50 µg/ml for mouse platelets. 4Mean fluorescence intensity (MFI) of CD62P expression on the surface of CD42a+CD61+ gated human or CD41+CD61+ gated mouse platelets was analyzed by FACS as described in Methods. Mean ± S.E. of five separate experiments is shown. 5***, P<0.001. 6*, P<0.05. 7Percentages (%) of FCShiSSChi aggregated CD42a+CD61+ gated human or CD41+CD61+ mouse platelets was analyzed by FACS as described in Methods. Mean ± S.E. of five separate experiments is shown. 8**, P<0.01.