nnm-2021-0286 - Supporting Information

<b>Figure S1.</b> The
TEM images of CuS nanoparticles
<b>Figure S2.</b> The
TEM images of CuS@mSiO2 nanoparticles.
<b>Figure S3.</b> The
size distribution histogram of CuS@mSiO2@MnO2 NCs
<b>Figure S4.</b> The
hydrodynamic size of CuS@mSiO2 nanoparticles.
<b>Figure S5.</b> The
surface potential of the CuS@mSiO2 nanoparticles.
<b>Figure S6.</b> The
signal to noise ratio (SNR) of tumor before and after CuS@mSiO2@MnO2
injection
<b>Figure S7.</b>
Radiosensitization effect of nanocomposites in vitro. (A) The relative cell
viability of Hela cells upon indicated treatments. 6Gy X-ray was applied. (B)
The surviving fraction of cells under incremental dose of X-ray irradiation
with or without NCs (C) The surviving fraction of cells after receiving
indicated treatments for 14 days after 6 Gy of X-ray irradiation. *
P&lt;0.01.
<b>Figure S8.</b> The
uptaken of DOX by cancer cell with or without X-ray irradiation. The
intensity of red fluorescence reflected the capacity of CuS@SiO2@MnO2 to
release DOX.
<b>Figure S9.</b> Statistical analysis of TUNEL staining in
different groups.
<b>Figure S10.</b>
The expressions of Ki67 were examined by immunohistochemistry, the mean
optical density is quantified by Image J software.
<b>Figure S11</b>.
The expressions of GPX4 in different groups were examined by
immunohistochemistry, the mean optical density is quantified by Image J
software.
<b>Figure S12.</b>
The biosafety of NCs and NCs/DOX (A) The representative images of major
organs in mice after injection with PBS, NCs, NCs/DOX, respectively.
<b>Table S1.</b>
Radiobiological parameters in different groups.
Abbreviations: D0
refers to the mean lethal dose; Dq refer to quasi-threshold dose; N
represents the extrapolation number; SER: sensitization enhancement ratios