nnm-2021-0286 - Supporting Information

<b>Figure S1.</b> The TEM images of CuS nanoparticles <b>Figure S2.</b> The TEM images of CuS@mSiO2 nanoparticles. <b>Figure S3.</b> The size distribution histogram of CuS@mSiO2@MnO2 NCs <b>Figure S4.</b> The hydrodynamic size of CuS@mSiO2 nanoparticles. <b>Figure S5.</b> The surface potential of the CuS@mSiO2 nanoparticles. <b>Figure S6.</b> The signal to noise ratio (SNR) of tumor before and after CuS@mSiO2@MnO2 injection <b>Figure S7.</b> Radiosensitization effect of nanocomposites in vitro. (A) The relative cell viability of Hela cells upon indicated treatments. 6Gy X-ray was applied. (B) The surviving fraction of cells under incremental dose of X-ray irradiation with or without NCs (C) The surviving fraction of cells after receiving indicated treatments for 14 days after 6 Gy of X-ray irradiation. * P&lt;0.01. <b>Figure S8.</b> The uptaken of DOX by cancer cell with or without X-ray irradiation. The intensity of red fluorescence reflected the capacity of CuS@SiO2@MnO2 to release DOX. <b>Figure S9.</b> Statistical analysis of TUNEL staining in different groups. <b>Figure S10.</b> The expressions of Ki67 were examined by immunohistochemistry, the mean optical density is quantified by Image J software. <b>Figure S11</b>. The expressions of GPX4 in different groups were examined by immunohistochemistry, the mean optical density is quantified by Image J software. <b>Figure S12.</b> The biosafety of NCs and NCs/DOX (A) The representative images of major organs in mice after injection with PBS, NCs, NCs/DOX, respectively. <b>Table S1.</b> Radiobiological parameters in different groups. Abbreviations: D0 refers to the mean lethal dose; Dq refer to quasi-threshold dose; N represents the extrapolation number; SER: sensitization enhancement ratios