Long-term co-culture induces reproducible transcriptomic changes not mediated by epigenetics in ovarian cancer cells: Highlighting the role of tumor microenvironment in therapy resistance.

Our study highlights the microenvironment's influence on ovarian cancer cells, showing how co-culture conditions with MSCs and ECs lead to distinct transcriptomic, genetic, and epigenetic alterations. These findings underscore the potential for targeting microenvironmental interactions to address tumor heterogeneity and treatment resistance