Differentially expressed cell proliferation-associated genes in AT-MSCs from three different donors, cultured to low or high density, as determined by microarray analysis.

Viable second-passage AT-MSCs plated at 200 cells/cm2 (CC1 MSCs) or 5,000 cells/cm2 (CC2 MSCs) were incubated for 7 days, by which time they reached ∼50% or ∼90% confluence, respectively. After harvesting, mRNA from three donor pooled samples of AT-MSCs was used in the microarray analysis. Microarray data were filtered by applying two criteria for significance, P<0.05 and FC>2 between culture conditions.