Next Generation Sequencing of expression profile from synchronized WT and BRD4 KO MEFs cells at different time point

Brd4f/f ERT2Cre MEFs are a type of fibroblast prepared from Brd4f/f ERT2Cre/- mouse embryo. MEFs are widely used in life science researches, especially in stem cell biology. Using RNAseq it was shown that many of cell cycle genes were under control of BRD4. Purpose of this study was to assess the effect of BRD4 KO in NGS derived transcriptome profiles of both WT and BRD4KO cells. RNA-seq from synchronized WT and BRD4 KO MEFs cells at different time point. Brd4f/f ERT2Cre MEFs were prepared from Brd4f/f ERT2Cre/- mice. mRNA profiles were generated from paired-end sequencing of duplicate samples using Illumina Hiseq 2000.