Determination of branch point (BP) usage by wild-type and mutant SF3B1 using a minigene library

We address define differential usage of branchpoint (BP) in by wild-type SF3B1 SF3B1-K700E using a synthetic mini-gene synthetic library with variable 3' spice sites (3'SS). Minigene-specific libraries were prepared and sequenced on the illumina platform to determine differnces in splice-site usage. Overall design: Minigene-based analysis of differential splicing