RNA and ATAC sequencing of BMDCs after stimulation via FcRgamma. Mus musculus

To understand the effects of Fc receptor gamma chain (FcRgamma) signaling on gene transcription, we examined chronological chromatin accessibility and gene expression using five conditions of bone marrow-derived dendritic cells (BMDCs): M. bovis Bacille de Calmette et Guerin (BCG) stimulation in signal molecule- or receptor-deficient cells; mannose-capped lipoarabinomannnan (LAM) or trehalose-6,6-dimycolate (TDM) stimulation; TDM stimulation via constitutively expressing Mincle; spleen tyrosine kinase (Syk) inhibitor-treated LAM stimulation; anti-CD8 antibody stimulation via CD8/FcRr-chimeric receptor. From all RNA and ATAC-seq data, we found that the kinetics of FcRgamma signaling is converted into distinct chromatin status and generates different gene expression profiles in dendritic cells.