Effect of TP53 missense mutation R172H on the single cell gene expression of pre-neoplastic mouse mammary gland

Mutations in the TP53 gene are the most common genetic alterations in breast cancer. To investigate at a single cell level what is the gene expression network that is regulated by mutant p53 at a non-tumoral stage, we employed a pre-neoplastic mouse model harboring the germline hotspot missense mutation R172H. We thus performed scRNAseq of cells derived from the normal mammary glands of mice harboring wt or mutant p53. Single cell-RNA seq of cells isolated from the normal mammary glands of one p53+/+ mouse (p53WT)and one p53R172H/R172H Ki mouse (p53MUT)