Combining XPCT and immunohistochemistry-type approach for identifying disease relevant cells in 3D brain environment:nanoXRF imaging for the

X-ray phase contrast tomography (XPCT) allows 3D visualization of disease-relevant vascular and neuronal networks within central nervous system tissue, but the technique has limitations in the univocal identification of disease-relevant cells. We intend to boost XPCT to a higher level equipping it with nanotechnology solutions by combining two nanocomponents, metal nanoparticles (NPs) and cell marker-specific nanobodies (single-chain antibodies) so providing detailed image of the whole organ, with imaging at the cellular level, identifying the relevant cells via an immunohistochemistry-type approach. We propose to perform Holo-tomography and Xray Fluorescence experiment to validate this new imaging approach. The data will be correlated with planned and performed above and below K-edge measurements.