Native elongating transcript sequencing (NET-seq), nascnet RNA-seq, and total RNA-seq of wild type and RNA polymerase II C-terminal domain mutants and ChIP-nexus of RNA polymerase II C-terminal domains phosphoisoforms and splicing factors in S. cerevisiae

Transcription controls splicing and other gene regulatory processes, yet mechanisms remain obscure due to our fragmented knowledge of the molecular connections between the dynamically phosphorylated RNA polymerase II (Pol II) C-terminal domain (CTD) and regulatory factors转录（Transcription）可调控剪接及其他基因调控过程，但目前其背后的分子机制仍不明朗。这是由于我们对动态磷酸化的RNA聚合酶II（RNA polymerase II，Pol II）C端结构域（C-terminal domain，CTD）与调控因子之间的分子关联，尚缺乏完整且系统的认知。