Induced pluripotent stem cells-derived GABAergic interneurons Transcriptome in Down syndrome. Homo sapiens

Down syndrome (DS), resulting from an extra chromosome 21 (HSA21), or trisomy 21, is the most common genetic disorder of intellectual impairment. The brain of Down syndrome (DS) patients exhibits fewer interneurons in the cerebral cortex but its underlying mechanism remains unknown. In order to gain insights into the molecular underpinning of DS GABAergic interneuron behaviors, we performed RNA sequencing using day-45 GABA interneurons that were differentiated from three independent Down syndrome patients (DS1, 2DS3, and DSP) and three euploid iPSCs (DS2U, IMR90-4, and H9). Gene expression profiling revealed altered cell migratory pathways in vitro.