The effect of Uphill and Downhill treadmill Running on the transcription of peripheral blood mononuclear cells in young male SD rats

Using Affymetrix GeneChip® Rat Genome 230 2.0 Array chip to screen the effect of Uphill and Downhill treadmill exercise on the key genes that affect bone mass in the peripheral blood mononuclear cells (PBMCs) of young male rats. Thirty-six male Sprague-Dawley rats were randomly assigned to a control group and both running groups of uphill and downhill (n = 12). Bone mineral density (BMD), bone mineral content (BMC) and bone microstructure of the femur were measured. PBMCs were extracted and the gene expression profile of mRNA detected.Gene Ontology and KEGG pathway analysis were used.The results showed exercise mainly activated the biological processes and cellular pathways involved in immune, inflammation and osteoclast differentiation. the effect of Uphill and Downhill treadmill exercise on the mRNA profiles in the peripheral blood mononuclear cells (PBMCs) of young male SD rats.