An insight into the sialomes of Psorophora albipes, Anopheles dirus and An. freeborni

Saliva of blood sucking arthropods contains a complex mixture of bioactive polypeptides that disarm their hosts blood coagulation, platelet aggregation and vasoconstriction. We here run barcoded cDNA from the salivary glands of adult female Psorophora albipes, Anopheles dirus and Anopheles freeborni on a single lane of a Hiseq Illumina sequencer. Sequences were assembled using Abyss and SoapdenovoTrans with several k values and the resulting contigs were further assembled by blast and cap3 iterations. There was some contamination between samples that were filtered out in the final results.