Long-range enhancer-controlled genes are hypersensitive to regulatory factor perturbations Tjalsma et al.

To study the contribution of regulatory factors to long-range enhancer-mediated gene regulation, we created degron cell lines for the factors RAD21, LDB1, and MED23, in human K562 cells. By endogenously tagging these proteins with a degron tag, we were able to quickly deplete them and measure their effect on gene expression using nascent RNA-sequencing. These files contain raw western blot images used to confirm that treatment of these cells with the degron ligand resulted in depletion of the tagged factors.