data used to create main figures for: Park et. al., Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA, BMC Biol, 2023.
The goal of the experiment is to identify genes involved in T-cell receptor (TCR) signaling and T-cell activation in human T cells. The Jurkat E6 cell line, engineered to express dCas9-KRAB and transd
We compared changes in sgRNA abundance between T24 cells challenged and unchallenged by CD8+ T cells, and then performed negative screening (p < 0.01, log2Fold change < −1.0, and good sgRNAs ≥ 5). (X
Investigating Bcor target genes in BcorΔE9-10KrasG12D leukaemia with a custom CRISPR dropout screen BcorΔE9-10KrasG12D cell line was transduced with a custom CRISPR library and a dropout screen was pe
Human pluripotent stem cells (hPSCs) provide an invaluable tool for modeling diseases and hold promise for regenerative medicine. For understanding pluripotency and lineage differentiation mechanisms,
