IPK soil 16S rRNA sequencing

The raw sequence reads submitted belong to 16S rRNA pyrosequencing (Roche 454) from DNA chronically contaminated soil samples treated with different concentrations of the surfactant Triton X-100 and the control without it. Triton X-100 was used as a biostimulation strategy to bioremediate the contaminated soil by solubilizing the polycyclic aromatic hydrocarbons sorbed in the soil matrix. The use of pyrosequencing—a powerful tool for analyzing complex microbial communities—in combination with the chemical determination of the sorbed and bioavailable PAH fractions enabled us to obtain strong evidence for the involvement of both physicochemical and biologic influences on the efficiency of the process.