Hepatoblastomas with carcinoma features represent a biological spectrum of aggressive neoplasms in children and young adults II

We molecularly characterized a class of histologically aggressive childhood liver cancers and showed that these tumors are clinically aggressive and that their observed histological features are associated with underlying recurrent molecular features. We proposed a diagnostic algorithm to identify these cancers using a combination of histological and molecular features, and our analysis suggested that these cancers may benefit from specialized treatment strategies that may differ from treatment guidelines for hepatoblastomas and hepatocellular carcinomas. Genome-wide copy numbers were estimated using DNA isolated from fresh frozen and formalin-fixed paraffin-embedded (FFPE) tumor tissue (38 cases) using the Affymetrix OncoScan FFPE Assay Kit and OncoScan Console 1.3, and data were analyzed by Affymetrix CHAS 3.1 and OncoScan Nexus Copy Number 7.5 after BWA alignment to GRCh37/hg19.