A k-mer-based approach for phylogenetic classification of taxa in environmental genomic data

In the age of genome sequencing, whole genome data is readily and frequently generated, leading to a wealth of new information that can be used to advance various fields of research. New approaches, such as alignment-free phylogenetic methods that utilize k-mer-based distance scoring, are becoming increasingly popular given their ability to rapidly generate phylogenetic information from whole genome data. However, these methods have not yet been tested using environmental data, which often tends to be highly fragmented and incomplete. Here we compare the results of one alignment-free approach (which utilizes the D­2 statistic) to traditional multi-gene maximum likelihood trees in three algal groups that have high-quality genome data available. In addition, we simulate lower-quality, fragmented genome data using these algae to test method robustness to genome quality and completeness. Finally, we apply the alignment-free approach to environmental metagenome assembled genome data of uncla..., ,