TRIM28 modulates progesterone and estrogne signaling in endometrium [ATACseq-hg38]

TRIM28 interacts with PGR and ESR1 in both human and mouse uterus to modulate estrogen and progesterone signaling. Knocking down of TRIM28 in the human endometrial stromal cells imparied decidualization in vitro. Deletion of TRIM28 from mouse uterus disrupted uterine stromal decidualization leading to infertility. Addtionally, TRIM28 deletion caused abnormal accumulation of TRIM28 postive and PGR negative cells in the stroma. Overall design: Primary human endometrial stromal cells from three donors were treated with either non-targeting siRNA (siNT) or TRIM28 siRNA (siTRIM28) for 48h then treated with either vehicle or EPC (17beta-estradiol, progesterone and cAMP) for 72h to generate pre-decidual (Pre-dec) and decidaul (Dec) cells and collected for ATAC-Seq analysis.