Lipidomic dataset of MDA-MB-231 human breast cancer cells treated with diacylglycerol acyltransferase (DGAT) inhibitors and docosahexaenoic acid (DHA)

Lipid droplets (LDs) are dynamic fat storage organelles involved in fatty
acid metabolism. By storing polyunsaturated fatty acids (PUFAs) in the
form of neutral lipids, LDs can either mitigate or exacerbate lipotoxic
damage. However, their role in regulating cellular fatty acid
distribution, membrane unsaturation, and ferroptosis susceptibility
remains poorly understood. To explore how LD turnover modulates membrane
lipid composition and ferroptosis sensitivity in cancer cells exposed to
exogenous PUFAs, MDA-MB-231 human triple-negative breast cancer cells were
supplemented with non-lethal concentrations of exogenous docosahexaenoic
acid (DHA) and treated with inhibitors of diacylglycerol acyltransferases
(DGAT) 1 and 2. Untargeted lipidomic analysis revealed substantial
lipidome remodeling with 302 significantly altered lipids in response to
DGAT inhibition, DHA supplementation, or combined treatment.