Microcystis aeruginosa PCC7806 proteomics

Triplicate cultures of Microcystis from the indicative time points were collected for proteomic analysis. Proteins were extracted, quantified, and digested using the previously described method. The samples were reconstituted with mobile phase A (2% ACN, 0.1% FA), centrifuged at 20,000 g for 10 min, and the supernatant was taken for loading. Separation was carried out by a Shimadzu LC-20AB liquid system equipped with a 5 um, 4.6 × 250 mm Gemini C18 column at a flow rate of 300 nL/min. After being separated by a non-linear gradient of mobile phase B (98% ACN, 0.1% FA), the end of the nanoliter liquid phase separation was directly connected to the timsTOF Pro spectrometer (Bruker, Germany) for mass spectral analysis.