Single-cell sequencing of protoplasts from the Physcomitrium patens after blue light and white light treatment (PRJCA041432)

This project explores the molecular mechanisms by which blue light enhances cellular reprogramming in Physcomitrium patens protoplasts , with a focus on the role of SHMT1(Serine Hydroxymethyltransferase 1). Through single-cell RNA sequencing (scRNA-seq) of protoplasts and leaf tissues exposed to blue light versus white light, we identified SHMT1 as a key upregulated gene under blue light. Functional analysis suggests that SHMT1-mediated metabolic shifts promote cell fate plasticity, providing insights into light-regulated reprogramming pathways in bryophytes. This study bridges photobiology with single-cell omics, offering potential applications in plant synthetic biology.