Linear regression models assessing the impact of combination of assay conditions on assay variability (log10MAD of log10MFI).

Regression coefficients and p-values for one and two factors, all dilutions and antigens included. Bead coupling performed once at the beginning of the study (stock) or three times along the study (several); Sample predilution prepared at once (stock) or daily (daily); Sample-bead incubation temperature at 22°C or 37°C; Plate washing in an automated washer (automatic) or manually (manual); and Operator expertise, apprentice or expert. A) Positive control, 2 conditions, 8 dilutions and 7 antigens (N = 112); B) Subject samples, 2 conditions, 4 dilutions and 7 antigens (N = 56) and C) Multiplex blanks, 2 conditions and 7 antigen (N = 14). P-values adjusted for multiple testing by Benjamini & Hochberg (p-Adj). Significant p-values are shown in bold.