Data underlying the publication: Mapping recombination landscape and basidial spore number in the button mushroom Agaricus bisporus

- Raw data of counts of basidial spore numbers in lamellae of 2 varieties of A. bisporus, i.e. var. bisporus and var. burnettii (parents).<br>The intervarietal hybrid and the intercross of haploid offspring of the intervarietal hybrid. These data sets are used to map QTL of the basidial spore number.<br>- A selection of 71 haploid offspring of HBT03 that was outcrossed with H39. This set was used to generate 40-50 haploid offspring that was subsequently genotyped to assess crossover positions.<br>These data were used to QTL map the trait " recombination landscape"<br>- Genotypes of the intervarietal hybrid HBT03 (var. bisporus H97 x var. burnettii H119p4).<br>180 homokaryotic single spore isolates (SSIs) were genotyped using 215 SNP's using Kasp genotyping method.<br>- 71 haploid offspring of the intervarietal hybrid HBT03 were outcrossed with the var. bisporis homokaryon H39.<br>From each hybrid, 40-50 haploid offspring was genotyped using 4 SNP markers per chromosome:<br>markers at the chromosome ends, and 2 markers 150-200 kb from chromosome ends ("border" marker).<br><br>

本数据集包含双孢蘑菇（Agaricus bisporus，缩写A. bisporus）两个变种的菌褶担孢子数量计数原始数据，两个变种分别为原变种var. bisporus与伯内特变种var. burnettii，二者为该实验的亲本。此外还包含种间杂交菌株及其单倍体后代的互交实验数据，上述数据集可用于开展担孢子数量性状的数量性状位点（Quantitative Trait Locus, QTL）定位分析。
- 选取由HBT03与H39互交获得的71株单倍体后代作为实验材料，该群体用于衍生40~50株单倍体后代，后续通过基因分型实验以评估染色体交换位点的位置。相关数据被用于开展"重组景观"性状的QTL定位分析。
- 种间杂交菌株HBT03的基因型数据，该菌株由双孢蘑菇原变种H97与伯内特变种H119p4杂交获得。采用Kasp基因分型技术（Kasp genotyping）对180株同核体单孢子分离株（Single Spore Isolates, SSIs）进行215个单核苷酸多态性（Single Nucleotide Polymorphism, SNP）位点的基因分型。
- 将种间杂交菌株HBT03的71株单倍体后代与双孢蘑菇原变种同核体菌株H39进行互交。针对每株杂交菌株，选取40~50株单倍体后代，利用每条染色体上的4个SNP标记完成基因分型：其中2个标记位于染色体末端，剩余2个标记距染色体末端150~200 kb，即"边界标记"。