Relative binding of Mamu-A1*00201 tetramers folded with four different SIV peptides to seven allotypes of Mamu-KIR3DL05.

Jurkat cells were transfected with constructs expressing HA-tagged allotypes of Mamu-KIR3DL05 and stained the next day with one of the following Mamu-A1*00201 tetramers; Gag71-79 GY9, Env788-795 RY8, Nef159-167 YY9 or Vif89-97 IW9. The cells were then stained with a monoclonal antibody to the HA tag and analyzed by flow cytometry. Values represent the MFI of tetramer staining divided by the MFI of HA staining for the transfected, eGFP+ cell population. Values were not calculated if the number of tetramer-positive events was less than 1000.