G-quadruplex in the gene of the large subunit of plant RNA polymerase II: billion years old story

<strong>Supplementary material to the journal article</strong> Consist of: Supplementary material S1: Analyzed <em>RPB1 </em>sequences in 40 plant species together with detailed characteristics and G-quadruplex prediction using four different computational approaches. Supplementary material S2: G4 locus is the most conserved within the <em>RPB1</em> gene (40 bp long potential G4 locus is the most conserved site in the whole ~ 6000 bp long <em>rpb1</em> gene. See the histogram below the alignment: the position of the G4 locus is depicted, together with the horizontal red dashed line indicating relative nucleotide conservation among aligned sequences of<em> RPB1</em>) Supplementary material S3: Multiple alignment of G4 locus of <em>RPB1</em> paralogs in <em>Arabidopsis thaliana </em>centered to G4 locus of <em>RPB1 </em> Supplementary material S4: Modelled 3D structure of G4 from <em>Bathycoccus prasinos</em> in PDB format Supplementary material S5: Gel electrophoresis and ThT staining of the selected G4-forming sequences Supplementary material S6: All analyzed <em>RPB1</em> sequences in FASTA format Supplementary material S7: Aligned <em>RPB1</em> sequences in FASTA format Supplementary material S8: <em>RPB1</em> paralogs in <em>Arabidopsis thaliana</em> Supplementary material S9: Spectral composition of light used in the UV experiment. Analysis of emitted light was performed by Ocean Optics (HR4000CG-UV-NIR, USA) device. Supplementary material S10: Difference CD spectra - comparison without and with previous UV treatment