Kobuvirus time-scaled tree mapping with host traits

Maximum Clade Credibility (MCC) tree representing the time-scale phylogeny of kobuvirus from all hosts based on the VP1 gene (n=416). Tree was genereated using Beast V1.8.2.The best fitting model was a general time-reversible (GTR) model with a gamma distribution (G) across sites as the substitution model, with an uncorrelated log-normal relaxed molecular clock model and with a constant size coalescent process prior over the phylogenies. The MCMC chains were run for 100 million iterations with sub-sampling every 10,000 iterations and 10% burn-in.we applied a strong prior of substitution rate using a normal distribution (mean rate = 2×10-3 substitutions/site/year; Standard Deviation =5×10-4).An asymmetric model with Bayesian Stochastic Search Variable selection (BSSVS) was applied to identify statistically significant transition rates between host species and geographic locations.The tree file was viewed and edited in Figtree V1.4.2.