N-4YSyn-induced cytotoxicity of stimulated T cells.

aAnti-CD3 stimulated T cells were cultured for 24 hrs in media alone or in the presence of different concentrations of 4YSyn or N-4YSyn, stained with 2 µg/ml of the vital dye, PI, washed, and assessed by flow cytometric analysis for uptake of PI.
bPercentage of T cells susceptible to PI permeation as determined by flow cytometric analysis.
cMean fluorescence intensity of PI-stained T cells.
dD statistic at α = 0.001 of Kolmogorov-Smirnov (K-S) analysis for fluorescence intensities of PI-stained T cells as the sigmoidal function of the accumulated cell frequency curve and fluorescence intensity (channel number) compared to the curve of PI-stained T cells from the medium control (asterisk) as computed by Cellquest software (BD Biosciences) [88].
eIndex of similarity = D/[(nc+nt)/(nc·nt)]1/2, where nc and nt are the number of events in cell frequency curves for medium control (c) and test substance (t), respectively (BD Biosciences) [88]. An index of similarity = 0 indicates the curves are identical.