Gene expression profiling of sensory epithelium of cochleas and vestibules of the inner ears of wild-type C57Bl/6J mice at post-natal day 0 (P0)

The sensory epithelium of cochleas and vestibules of mice were compared. The two tissues are quite similar in structure, but have distinct roles in hearing and balance. By comparing their gene expression, we hoped to identify key regulators of differentiation. Cochlear and vestibular sensory epithelium was dissected from 20 inner ears of 10 P0 C57Bl/6J mice, generating 2.4 and 1.5 ug of total RNA, respectively. 450 ng RNA from each sample was used to create libraries with the TruSeq Stranded mRNA Sample Prep Kit (Illumina), followed by high-throughput sequencing at 100 bp paired end (PE) at the Technion Genome Center, Haifa, Israel. Six samples were generated, 3 cochlear and 3 vestibular, for sequencing in triplicate.本研究对小鼠耳蜗与前庭的感觉上皮开展比较分析。两种组织的结构高度相似，但在听觉感知与平衡调控中发挥着截然不同的生理功能。本研究通过对比二者的基因表达谱，旨在鉴定其分化过程中的关键调控因子。 实验材料取自10只出生后0天（P0）的C57Bl/6J小鼠的20个内耳，分别解剖分离得到耳蜗感觉上皮与前庭感觉上皮，二者的总RNA产量依次为2.4 μg与1.5 μg。每份样本取450 ng总RNA，使用TruSeq链特异性mRNA样本制备试剂盒（TruSeq Stranded mRNA Sample Prep Kit，Illumina）构建测序文库，随后于以色列海法的Technion基因组中心开展100 bp双端（PE）高通量测序。本实验共设置6个测序样本，耳蜗与前庭样本各3份，均进行三次生物学重复测序。