Cytokine-secretion and Granzyme B response to TcCA-2 epitopes by PBMCs from HLA-A*02:01 Chagas disease patients and healthy donors.

a HLA-A*02:01 Chagas disease patients in asymptomatic phase (IND) and cardiac forms (CCC). HD: HLA-A*02:01 healthy donors. The cytokine secretion and the number of GzB-secreting cells were calculated by subtracting background levels or the number of spots in control wells (non-peptide) from the levels measured or the number of spots obtained in the stimulated cultures. Responses were considered positive (in bold) when:b The cytokine concentration is at least three folds the non-peptide control value and ≥15 pg/mL for IL-6 and ≥ 5 pg/mL for IFN-γ and TNF-α.c The number of SFC per million was ≥20 and at least twice the value found in the background control wells.Cytokine-secretion and Granzyme B response to TcCA-2 epitopes by PBMCs from HLA-A*02:01 Chagas disease patients and healthy donors.