Next-generation sequencing of Tfh cells from CSF compared to paired blood in multiple sclerosis

Tertiary lymphoid structures are reported in the meninges of patients with multiple sclerosis, especially at the progressive stage and are strongly associated to cortical lesions and disability. Besides B cells, these structures comprise follicular helper T cells (Tfh) that are crucial to support B cell differentiation. Tfh play a pivotal role in amplifying autoreactive B cells and promoting autoantibody production in several autoimmune diseases but very few is known in multiple sclerosis. Here we reporter transcriptomic profile of CSF infiltrating Tfh compared with paired blood in RRMS patients. Overall design: RNAseq analysis of Tfh from CSF and Tfh from blood in multiple sclerosis patients