Profiling the spatial architecture of multiple myeloma in human bone marrow trephines with spatial transcriptomics [human]

Here, we used subcellular spatial transcriptomics to profile the expression of 5,001 genes in human bone marrow in the context of monoclonal gammopathy of undetermined significance, smouldering myeloma and multiple myeloma. Using this approach, we explored the plasma cell and stroma ecosystem in bone marrow trephines from 21 individuals including 7 with pre-malignant disease and 10 with active multiple myeloma. Xenium profiling on bone marrow trephines.