Single-nucleus single-nucleus assay for transposase-accessible chromatin with sequencing analysis of in vitro cardiac differentiations

Here, we utilize single-cell gene regulatory analysis to interrogate the gene expression within CMs generated from monolayer (2D) and embryoid body (3D) hPSC cardiac differentiation systems. Overall design: We performed differentiations into 2D and 3D CM systems, and collected cells at six distinct stages for snATAC-seq collection.