Fate of the secretory organelles and mitochondrial division in dd-Rab11B_N125I expressing parasites.

(A,B and C) Immunofluorescent analysis of ddRab11BN125I expressing parasites incubated in absence and presence of Shld-1 for 24 h and stained with the indicated antibodies. In presence of Shld-1 the organelles are still formed, although the organelles lose their apical localisation (see also Figure 7, 8). SAG1 appears to be correctly localised to the plasma membrane of the mother parasite indicating functional transport of GPI-anchored proteins to the surface.(D and E) ddRab11BN125I parasites co-expressing the mitochondrial marker HSP60-RFP (red) or the apicoplast marker FNR-RFP (red) were treated without and with Shld-1 for 24 h and subsequently labelled with anti-IMC1 (green). In presence of Shld-1 mitochondrial division and apicoplast segregation appeared normal. Scale bars represent 5 µm.