The original SDS/PAGE immunoblot for LAM analysis in H37Rv and ΔlprG cellular extracts.
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https://figshare.com/articles/dataset/Correction_LprG_Mediated_Surface_Expression_of_Lipoarabinomannan_Is_Essential_for_Virulence_of_Mycobacterium_tuberculosis_/1622617
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Extracts normalized to protein concentration were separated on a 15% SDS/PAGE gel and transferred to PVDF membrane. The blot was blocked, and then stained with anti-LAM pAb (α-LAM) followed by goat anti-rabbit IgG-HRP secondary antibody. The blot was washed and imaged after adding 30% 3,3’-diaminobenzidine tetrahydrochloride solution plus 0.0005% H2O2. LAM Std, purified H37Rv LAM standard. Soluble cell wall, purified H37Rv cell wall extract. (PPTX)
创建时间：
2015-12-11
