Expression data from primary human lung MVPC

MVPC isolated from explanted lung tissue at VUMC.; Control MVPC - 3 independent patients, mTOR activated MVPC - 3 lines 1 patient; Fetal 3 independent patients. Fetal lung MSC 3 independent patients LAM MVPC - 1 patient 3 different parts of lung; Fetal lung MVPC 3 independent patients; Control MVPC - 3 independent patients To elucidate pathways in human MVPC that were affected by mTOR activation, transcriptome expression analysis via microarray comparing primary human lung MVPC from normal control, mTOR+ and fetal lung was performed. Following normalization, hierarchical clustering analyses were presented as a heatmap (Figure 2A; Supplemental Figure 4), illustrating significant differences between the mTOR activated MVPC and normal controls. Interestingly, mTOR activated MVPC patterns of gene expression overlapped approximately 33% with the fetal lung MVPC Human Affy 1.0 st chips; CU AMC Genomics Core 2016