Single cell ATAC-seq of mouse hair follicle morphogenesis

Mouse, also known as a good model animals, is widely uaed in the research of hair follicle development. Unraveling the dynamic accessibility of regulatory chromatin at single-cell resolution is key to understanding stem/progenitor cell fate choices. and it's therefore of significance to investigate the chromatin accessibility and transcriptional regulation mechanism during mouse hair follicle development. To provides comprehensively understanding on the transcriptional regulation mechanism and cell lineage cell fate decisions, we performed single-cell ATAC sequencing on 23,716 single cells from induction (embryonic day 13.5), organogenesis (embryonic day 16.5) and cytodifferentiation (postnatal day 0) stage fetus mouse dorsal skin. Based on unsupervised clustering analysis, cell lineage inference and etc, the single cell map of chromatin open area was drawn from each cell type and the mechanism of cell transcription regulation was explored. Collectively, our data here provide a reference for deeply exploring the epigenetic regulation mechanism of mouse hair follicles development. We performed single-cell ATAC sequencing on 23,716 single nuclear samples from induction (embryonic day 13.5), organogenesis (embryonic day 16.5) and cytodifferentiation (postnatal day 0) stage fetus C57BL/6 mouse dorsal skin.