Effects of the treatment with GA on the CCl4-induced liver fibrosis and lipid peroxidation.

The mice were treated with GA, silymarin or vehicle every day for 4 weeks on the other day of the first double-dosage administration with CCl4. The CCl4 dissolved in corn oil (6.4 g/kg of body weight, s.c.) was administrated under the skin between head and neck to each group every 6 days during this month, except Cont. The animals were sacrificed 24 h after the last CCl4 administration. Hepatotoxicity was determined by quantifying the serum activities of GPT and GOT as well as hepatic lipid peroxidation. Cont, normal control; CCl4, CCl4 alone; Sil, CCl4+100 mg/kg silymarin; GA25, CCl4+25 mg/kg GA; GA50, CCl4+50 mg/kg GA; GA100, CCl4+100 mg/kg GA. Data are presented as the mean±SD (n = 8) in each group.aSignificantly different from the control at pbSignificantly different from the CCl 4 at p