Expression data from intestinal mucosa of patients with Crohn disease

We used microarrays to detail the global signature of gene expression underlying endoscopic recurrence of CD and identified distinct gene signature predicting postoperative recurrence. Ileal samples from Crohn's disease patients and healthy samples from non-inflammatory controls were collected for RNA extraction and hybridization on Affymetrix microarrays. Inclusion criteria were age >18 years, ileal or ileocolonic CD and indication of CD‐related intestinal surgery. Endoscopic recurrence was defined by the presence of any ulcerated lesions at the anastomosis and/or on the neo-terminal ileum (Rutgeerts score > i0). Five hundred and twenty samples (520) were collected from the inflamed ileum (M0I) and the ileal margin (M0M) at time of surgery, and during post-operative endoscopy six month later (M6). We also collected 25 ileal non-IBD control biopsies (Ctrl) from patients who underwent ileocecal resection for colonic tumour with a healthy ileum.