Orchestrating endothelial cell fate regulation and function during multiple organs' development, stress and aging at single cell resolution

CoBATCH-supported single cell protein-bound elements profiling five tissues from Cdh5+ lineage endothelial cells (EC) collected from four stages of the mouse lifespan, discovering that intrinsic epigenetic plasticity grammarred by the TF regulome which educated by tissue microenvironment. Overall design: We applied CoBTACH to comprehensively trace Cdh5+ cells lineage for various histone modifications and TFs, obtaining total ~20,000 cells derived from 5 organs (heart, liver, lung, brain, kidney) across the lifespan of mouse. Each sample represents a single cell.