Untargeted metabolomics between ALDOB KO or WT mice exposed to control or 10% fructose solution
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Hydrophilic Interaction Liquid Chromatography (HILIC) was used to successfully separate isobaric hexose phosphate species, as previously described. Our data revealed that F1P was the most significantly altered metabolite in ALDOB KO mice upon fructose exposure. Interestingly, sorbitol levels were also increased suggesting that the polyol pathway (formation of fructose from glucose via sorbitol) is active in this setting.
