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Transcriptome analysis of wt1b:eGFP positive macrophages in the regenerating zebrafish heart

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NIAID Data Ecosystem2026-04-25 收录
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Little is known about zebrafish macrophage subtypes and their contribution to organ regeneration. Using the transgenic line wt1b:eGFP we identified a subtype of macrophages in the regenerating heart that is transcriptionally different from the rest of macrophages. Overall design: Hearts from Tg(wt1b:eGFP; mpeg1:mCherry) zebrafish were cryoinjured and at 4 dpi mCherry and GFP/mCherry cells were FAC sorted. RNA was extracted from 4 pools of GFP/mCherry and 4 pools of mCherry single positive cells and RNAseq was performed.

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2019-10-30
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